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Iipropathi kunye nokusetyenziswa kwekhabhoni esebenzayo

Ikhabhoni esebenzayo: luhlobo lweentengiso ezingezizo polar ezisetyenziswe ngakumbi. Ngokubanzi, kufuneka ihlanjwe nge-hydrochloric acid, ilandelwe yi-ethanol, emva koko ihlanjwe ngamanzi. Emva kokumisa kwi-80 ℃, inokusetyenziselwa ikholam chromatography. Ikhabhoni esebenzayo yecarular yeyona khetho ikhethekileyo kwikholam chromatography. Ukuba ngumgubo ocolekileyo wekhabhoni, kufuneka ukongeze inani elifanelekileyo le-diatomite njengoncedo lokucoca ulwelo, ukuthintela ukuhamba kancinci kakhulu.
Ikhabhoni eyenziweyo yintengiso ye-non-polar. I-adsorption yayo ichasene ne-silica gel kunye ne-alumina. Inobudlelwane obomeleleyo bezinto ezingezizo polar. Inesikhundla esomeleleyo se-adsorption kwisisombululo samanzi kwaye ibuthathaka kwisinyibilikisi sezinto eziphilayo. Ke ngoko, amandla okuphuma kwamanzi ngawona abuthathaka kwaye isinyibilikisi somzimba somelele. Xa into ekwi-adsorbed ikhutshiwe kwi-carbon eyenziwe yasebenza, ubunyibilikisi besinyibilikisi buyancipha, kunye nomthamo we-adsorption we-solute kwi-carbon esebenzayo iyancipha, kunye namandla okuphucula i-eluent ayonyuswa. Izinto ezinamanzi anyibilikayo, ezinje ngeamino acid, iswekile kunye neglycosides, zazizimele zodwa.


Ixesha Post: Nov-05-2020