Ikhabhoni esebenzayo: luhlobo lwe-adsorbent engeyo-polar esetyenziswa ngakumbi. Ngokuqhelekileyo, kufuneka ihlanjwe nge-dilute hydrochloric acid, ilandelwa yi-ethanol, kwaye ihlanjwe ngamanzi. Emva kokumisa kwi-80 ℃, ingasetyenziselwa ikholamu yechromatography. I-Granular activated carbon lolona khetho lungcono kwikholamu yechromatography. Ukuba ngumgubo ocolekileyo wekhabhoni esebenzayo, kuyafuneka ukongeza isixa esifanelekileyo sediatomite njengoncedo lokucoca, ukunqanda isantya sokuhamba kancinci.
Ikhabhoni esebenzayo yinto engeyiyo i-polar adsorbent. I-adsorption yayo ichasene ne-silica gel kunye ne-alumina. Inobudlelwane obuqinileyo kwizinto ezingezizo ezipolar. Inowona mthamo unamandla we-adsorption kwisisombululo samanzi kwaye ubuthathaka kwi-solvent ye-organic. Ke ngoko, umthamo we-elution wamanzi ngowona ubuthathaka kwaye isinyibilikisi se-organic sinamandla. Xa i-adsorbed substance ikhutshiwe kwikhabhoni evuliweyo, i-polarity yesinyibilikisi iyehla, kwaye umthamo we-adsorption we-solute kwikhabhoni esebenzayo uyancipha, kunye nomthamo we-lution we-eluent uyaphuculwa. Amalungu anyibilikayo emanzini, afana ne-amino acids, iiswekile kunye ne-glycosides, zaye zodwa.